Abstract:
The current progresses of single-cell sequencing and spatiotemporal transcriptome enable scientists not only can discover the specific cell types in tissue, but also can detect the cell distribution in the spatial dimension. Taking advantages of a newly developed spatial-temporal transcriptomics approach—SpaTial Enhanced REsolution Omics-sequencing (Stereo-seq) by BGI-research, with the highest profiling resolution to data, we studied the cellular and molecular dynamics during organ regeneration process in Axolotl, an animal that can regenerate damaged appendages and multiple internal organs, including the brain. Employing Stereo-seq, we generated a group of spatial transcriptomic data of telencephalon sections that covered six developmental and seven injury-induced regenerative stages. We discovered a sub-population of progenitor cells may then proliferate to cover the wound area and subsequently replenish lost neurons. Interestingly, we also observed that regeneration of the axolotl telencephalon exhibited similar neurogenesis patterns to development, suggesting that brain regeneration partially recapitulates the development process. Our spatial transcriptomic data highlights the cellular and molecular features of the axolotl telecephalon during development and injury-induced regeneration.
Venue:
Amphitheatre
Level 2, Duke-NUS
Host:
Asst Prof Alfred Sun
Principal Investigator
Neuroscience & Behavioural Disorders Programme
Duke-NUS
Contact Person:
Joyceline Ng (joyceline.ng@duke-nus.edu.sg)
Neuroscience & Behavioural Disorders Programme
Duke-NUS
Date and Time
13 Mar 2024 @ 00:00 - 13 Mar 2024 @ 00:00
Speaker
Dr Ying Gu
Principal Investigator
Deputy Director of BGI – Research
Beijing Genomics Institute
Ying Gu, Ph.D., Principal Investigator, and Deputy Director of BGI - research. She is also a member of the Anti-Aging Branch of the Chinese Geriatrics and Gerontology Society. Her main research areas include cell and molecular biology, developmental biology, stem cells and gene editing technology, as well as single-cell genomics. Using techniques such as single-cell and spatial genomics, she conducts scientific research in the fields of species chimerism, embryonic development, and regenerative mechanisms to analyze the differences in early embryonic development among different species, uncover the competition mechanisms of species chimerism, and decipher the molecular and cellular dynamic processes of limb and brain regeneration in salamanders. She led her team to publish a cover research article on salamander brain regeneration in Science, and has produced a series of achievements in journals such as Nature, Cell Stem Cell, Cell Discovery, Developmental Cell, Protein & Cell, providing important theoretical foundations for developmental and regenerative medicine research.