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Lena Ho Wai Mun

Associate Professor, Signature Research Programme in Cardiovascular & Metabolic Disorders

Duke-NUS Medical School

Bio

Dr Lena Ho is an Assistant Professor in the Cardiovascular and Metabolic Disorders program at Duke-NUS Medical School. She obtained her PhD at Stanford, where she studied the function and mechanism of chromatin remodelling proteins in the epigenetic regulation of embryonic, hematopoietic and cancer stem cells. During her postdoctoral training with Brun Reversade at A*STAR, she was one of the first to uncover the paradigm that non-coding RNAs can in fact encode microproteins from cryptic small open reading frames (sORFs). This is exemplified by the peptide hormone ELABELA encoded by what was thought to be a non-coding RNA. Starting with just a gene annotation, her work led to a comprehensive understanding of ELABELA’s molecular function as a hormone with pleiotropic effects on stem cell maintenance,
angiogenesis, and mammalian pregnancy. In 2017, Lena established the Endogenous Peptides Lab in Duke-NUS, which utilizes a combinatorial platform to discover and characterize novel sORF-encoded peptides in the human genome. Using ribosome profiling, the Ho lab has defined a mini proteome consisting of over 7000 microproteins in both healthy and inflamed tissues. By surveying their sub-cellular locations, the lab has discovered that membranous organelles such as the
mitochondria are highly enriched for microproteins. By focusing on microproteins localized to the mitochondria, the lab has uncovered new regulators of oxidative phosphorylation, lipid, and amino acid metabolism. These mitochondrial microproteins function as subunits of the electron transport chain (ETC), or assembly factors required for ETC assembly, or interacting partners of metabolite transporters. In each case, by studying the molecular mechanism of each microprotein, the lab has uncovered new paradigms of mitochondrial biogenesis, stress sensing, quality control and homeostasis. Dr Ho’s overall goal is to uncover programmatic functions that sORF peptides might play, afforded by their special size, biochemical and genomic properties. Lena is an EMBO Young Investigator and a Howard Hughes Medical Institute International Research Scholar.

Education

Master of Science

Stanford University, United States

Doctor of Philosophy

Stanford University, United States

Bachelor of Science with Hons

University of Wisconsin-Madison, United States

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